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post:post-processing_toolbox [2019/11/15 17:23] – [Track format conversion] pseudomoaner | post:post-processing_toolbox [2023/03/07 22:18] (current) – [Event labelling] pseudomoaner |
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==== Population labelling ==== | ==== Population labelling ==== |
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Many experiments consist of mixtures of distinct populations of cells; these scripts allow you to automatically separate out and label these populations. | Many datasets consist of mixtures of distinct populations of objects; these scripts allow you to automatically separate out and label these populations. |
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* [[post:splitFluoPopulationsTracks.m]]: Splits tracks into populations based on fluorescence levels in two channels. | * [[post:splitFluoPopulationsTracks.m]]: Splits tracks into populations based on fluorescence levels in two channels. |
==== Event labelling ==== | ==== Event labelling ==== |
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Often, we are interested in the time at which a cell performs a particular action. These scripts will help you to detect these 'events' | Often, we are interested in the time at which an object performs a particular action. These scripts will help you to detect these 'events' and assign them to FAST's internal representation. |
and assign them to FAST's internal representation. | |
| * [[post:labelThresholdEvents.m]]: Marks events when a specific track-associated variable drops above or below a specified threshold. |
| * [[post:labelUnusualEvents.m]]: Marks events when a specific track-associated variable moves far awy from its average, taking into account the typical fluctuations in its value. |
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==== Data manipulation ==== | ==== Data manipulation ==== |
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* [[post:meanIntInEllipse.m]]: Creates an elliptical mask based on the specified object's geometry and measures the intensity of an image within that mask. | * [[post:meanIntInEllipse.m]]: Creates an elliptical mask based on the specified object's geometry and measures the intensity of an image within that mask. |
| * [[post:filterTracks.m]]: Allows you to filter tracks with features (e.g. length, fluorescence intensity) that exceed or fall below a user-specified value. |
| * [[post:extractTimePoints.m]]: Allows you to switch from track to slice-based representation of a specified data field. |
| * [[post:coarsegrainTrackData.m]]: Converts motility data from track-based view to coarsegrained flowfield view. |
==== Lineage tree navigation and manipulation ==== | ==== Lineage tree navigation and manipulation ==== |
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The [[usage:division_detection|division detection]] module updates cell tracks to encode a lineage tree within them. These functions help manipulate and navigate this tree. | The [[usage:division_detection|division detection]] module updates tracks to encode a lineage tree. These functions help manipulate and navigate this tree. |
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* [[post: getLineageIndices]]: Allows you to get the indices and generations of all descendants of a target cell. | * [[post: getLineageIndices]]: Allows you to get the indices and generation numbers of all descendants of a target cell. |