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| usage:division_detection [2022/09/05 23:04] – pseudomoaner | usage:division_detection [2023/07/05 15:22] – [Division detection] pseudomoaner |
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| ===== Division detection ===== | ===== Division detection ===== |
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| Selection of the **division threshold** is also very similar to selection of the **adaptive linking threshold** in the tracking module, with the user repeatedly performing a cycle of threshold selection - division detection - validation - threshold update. However, unlike the tracking module, the relative sparseness of division events means that it is practical to perform threshold selection by repeatedly analysing the entire dataset, rather than a single pair of frames. Collating all data across all timepoints also means that a single threshold is applied for the entire dataset (rather than being varied automatically to account for changes in link ambiguity, as in the case of the tracking module). | In contrast to the Tracking module, the Division Detection module does not require the user to manually select a tracking threshold. Instead, the division detection algorithm automates selection of this threshold by iteratively modifying its value such that the resulting lineage structure is maximally connected while still remaining a tree (i.e. contains no loops). In practice, this generally gives a near-optimal balance between assigning true links - correct assignments between mother and daughter cells - and suppressing false links. |
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| To perform initial division detection, simply click the **Find divisions!** button. One of two outcomes will then occur: | |
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| - If division detection completed successfully, the division viewer and lineage distribution windows will update. | |
| - If division detection resulted in a lineage with a cycle (i.e. a cell marked as its own ancestor), the following warning notice will appear and the division detection process will be aborted: | |
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| {{ :usage:detectionwarning.png?nolink&400 |}} | |
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| In the case of scenario (2), the **division threshold** should be reduced until the warning notice ceases to appear. | |
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| | Once training has completed, it is therefore sufficient to simply press the **Find divisions!** button to launch this iterative algorithm and generate your lineage. |
| ==== Validation ==== | ==== Validation ==== |
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