usage:overlays

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usage:overlays [2019/08/06 17:00] – [Setting up the overlays] pseudomoanerusage:overlays [2022/09/05 23:04] (current) pseudomoaner
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 ===== Converting from frames to a movie ===== ===== Converting from frames to a movie =====
  
-Once you have triggered the bulk output, you will be able to access the set of generated frames in the new 'Overlays' folder in the root directory. You can now generate movies from these frames. There are many ways to do this, but two example methods are described below.+Once you are happy with the setup of the overlay, click **Run 'em all!** to apply these settings to all the frames in the dataset. When this has finished, you will be able to access the set of generated frames in the new 'Overlays' folder in the root directory. You can now generate movies from these frames. There are many ways to do this, but two example methods are described below.
  
 ==== Method 1: Using ImageJ/FIJI ==== ==== Method 1: Using ImageJ/FIJI ====
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 // //
  
-==== Event detection ==== 
  
 ==== Lineage tracking ==== ==== Lineage tracking ====
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 // //
  
-The 'generational age' of each cell in this dataset is also automatically calculated in the division detection module. Cells that die stop ageing, allowing them to be clearly seen in this movie. Cell ages are indicated with a coloured mask, starting from generation 1 (dark blue) and finishing at generation 6 (dark red).+The 'generational age' of each cell in this dataset is also automatically calculated in the division detection module. In this video, generated using the special 'Lineage trees' option, cell ages are indicated by the colour of the overlying track, starting from generation 1 (dark blue) and finishing at generation 6 (dark red).
  
 // //
  
-{{ youtube>rSnvglvt-rE?large }}+{{ youtube>AhCPggI5zz4?large }} 
 + 
 +// 
 + 
 +===== Video demonstration ===== 
 + 
 +{{ youtube>i3EOyxx05ss?large }}
  • usage/overlays.1565107241.txt.gz
  • Last modified: 2019/08/06 17:00
  • by pseudomoaner