Differences

This shows you the differences between two versions of the page.

--- post:labelstartandendfluocells.m [2019/10/11 15:26] – created pseudomoaner
+++ post:labelstartandendfluocells.m [2019/10/23 12:22] (current) – pseudomoaner
@@ Line 1: / Line 1: @@
 ====== labelStartAndEndFluoCells.m ======
-**Description:** Takes in the [[usage:tracking#outputs|procTracks]] data structure and two manually specified channel indices, and splits tracks based on the ratio of fluorescence in the two channels. Assumes that there are two populations in the dataset, each labelled in a separate channel.
+**Class:** Script
+**Dependancies:** [[post:meanintinellipse.m]]
+**Description:** Takes in the [[usage:tracking#outputs|procTracks]] data structure and a pair of fluorescence images, one taken at the beginning of the timeseries and one at the end. By only taking two fluorescence images, you can associate each of your tracks with a fluorescence-based population without causing excess phototoxicity or photobleaching.
+Fluorescent tracks are assigned to population 2. Non-fluorescent tracks are assigned to population 1. Tracks that cannot be assigned to either (as they overlap with neither the first timepoint nor the final timepoint) are assigned to population 3.
 **Author:** Oliver J. Meacock
@@ Line 59: / Line 65: @@
 </code>
-**Example output:** Plot generated in the [[usage:plotting]] module, showing how the two fluorescence channels are split into two separately labelled clusters:
+**Example output:** Plot generated in the [[usage:plotting]] module, showing how fluorescence can be used to separate a high motility (black) from a low-motility (red) genotype when mixed in a single system.
-{{ :post:fluosplitexample.png?nolink&400 |}}
+{{ :post:speedsplitexample.png?nolink&400 |}}