Have you struggled with automated measurement of the movement of many closely-packed individuals? The Durham lab's tracking system, FAST, may be the answer to your problems. Its features include:
Sound good? Download the latest distribution of the Matlab files here, the stand-alone version here, or see the installation guide for more details on how to perform the download and setup process.
The segmentation module allows you to automatically isolate individual objects from your images.
Feature detection quantifies properties of the objects detected by the segmentation module.
Tracking links together objects between timepoints. A sub-GUI also allows manual correction of tracks.
Many biological samples consist of cells that repeatedly divide. This module allows you to detect such divisions and reconstruct lineages.
Overlaying extracted data on top of raw images can provide powerful visualisations of your system.
The plotting module allows you to quickly assess relationships between features and variables extracted during tracking.
We are grateful for funding from the EPSRC and BBSRC, grant codes EP/F500394/1 and BB/R018383/1.