Yes, but you will first need to stitch your tile into a single, contiguous set of pixels for each channel at each timepoint. There are many tools to perform this task available on the web, such as ImageJ's Image Stitching plugin.

Yes. To do this, first split your dataset into each separate field of view and save each as an image file in a unique folder. For more details on how to set up your directory structure, please refer to the data configuration pages.